Modelling the assembly and flexibility of antibody structures

Curr Opin Struct Biol. 2023 Dec 19;84:102757. doi: 10.1016/j.sbi.2023.102757. Online ahead of print.ABSTRACTAntibodies are large protein assemblies capable of both specifically recognising antigens and engaging with other proteins and receptors to coordinate immune action. Traditionally, structural studies have been dedicated to antibody variable regions, but efforts to determine and model full-length antibody structures are emerging. Here we review the current knowledge on modelling the structures of antibody assemblies, focusing on their conformational flexibility and the challenge this poses to obtaining and evaluating structural models. Integrative modelling approaches, combining experiments (cryo-electron microscopy, mass spectrometry, etc.) and computational methods (molecular dynamics simulations, deep-learning based approaches, etc.), hold the promise to map the complex conformational landscape of full-length antibody structures.PMID:38118364 | DOI:10.1016/j.sbi.2023.102757
Source: Current Opinion in Structural Biology - Category: Biology Authors: Source Type: research