"Difficult" deoxyribonucleotide sequences in the solid-phase synthesis by the phosphoramidite chemistry

Nucleosides Nucleotides Nucleic Acids. 2023 Dec 20:1-9. doi: 10.1080/15257770.2023.2295478. Online ahead of print.ABSTRACTThis work catalogued oligonucleotide sequences and sequence compositions based on the overall yield of full-length product obtained by the phosphoramidite chemistry-based solid phase synthesis. In total, 76 sequences with different dinucleotide and trinucleotide repeats were synthesized, and the fully-deprotected products were analyzed by denaturing anion exchange HPLC. Overall, sequences containing more 2'-deoxyadenosine residues were obtained in relatively lower yields, likely due to the relative ease of 2'-deoxyadenosine to undergo depurination during the detritylation reaction. Furthermore, dinucleotide steps, such as d(CG)/d(GC) and d(AG)/d(GA), likely contribute the overall lower yields of full-length products as well.PMID:38116988 | DOI:10.1080/15257770.2023.2295478

Source: Nucleosides, Nucleotides and Nucleic Acids - December 20, 2023 Category: Biochemistry Authors: Quanjian Li Hongbin Yan Source Type: research