SELEX-discovered aptamer that inhibits cellular interleukin-17/interleukin-17 receptor interaction and antagonizes interleukin-17 signaling

Biosci Biotechnol Biochem. 2023 Nov 29:zbad170. doi: 10.1093/bbb/zbad170. Online ahead of print.ABSTRACTThis research is based on a systematic evolution of ligands by exponential enrichment (SELEX), also referred to as in vitro selection against the extracellular domain of human interleukin-17 receptor A (IL-17RA). Pull-down assay via quantitative polymerase chain reaction and chemiluminescence detection showed that the cloned RNA with the enriched sequence bound to human IL-17RA and inhibited the interaction between IL-17RA and human interleukin-17A (IL-17A). We also revealed that the newly discovered IL-17RA-binding RNA aptamer bound to cellular IL-17RA, inhibited the cellular IL-17RA/IL-17A interaction, and antagonized cellular IL-17A signaling.PMID:38031342 | DOI:10.1093/bbb/zbad170

Source: Bioscience, Biotechnology, and Biochemistry - November 30, 2023 Category: Biochemistry Authors: Arifur Rahman Munshi Tong Wang Yukio Takamori Takehiro Ando Takumi Yokoyama Daisuke Fuji Zhehao Xu Santhana Vedi Mizuki Yamamoto Keita Tsukamoto Takashi Kawakami Source Type: research