Effects of circulating endothelial microvesicles isolated from adults with obesity on endothelial cell inflammation, apoptosis and nitric oxide production

Am J Physiol Endocrinol Metab. 2023 Nov 22. doi: 10.1152/ajpendo.00139.2023. Online ahead of print.ABSTRACTCirculating endothelial-cell derived microvesicles (EMVs) have been shown to be elevated with obesity and associated with endothelial dysfunction; however, their direct effect on endothelial cells is unknown. The experimental aim of this study was to determine the effect of EMVs isolated from adults with obesity on endothelial cell inflammation, apoptosis, and nitric oxide (NO) production. EMVs (CD144+ microvesicles) were identified, enumerated and isolated from plasma by flow cytometry from 24 sedentary adults: 12 normal weight (8 M/4 F; age: 55±6 yr; BMI: 24.3±0.7 kg/m2; EMV: 144±53 EMV/µL) adults and 12 adults with obesity (6 M/6 F; 59±7 yr; 31.0±1.1 kg/m2; 245±89 EMV/µL). Human umbilical vein endothelial cells were cultured and treated with EMVs from either normal weight adults or adults with obesity. EMVs from obese adults induced significantly higher release of interleukin (IL)-6 (108.2±7.7 vs 90.9±10.0 pg/mL) and IL-8 (75.4±9.8 vs 59.5±11.5 pg/mL) from endothelial cells vs EMVs from normal weight adults, concordant with greater intracellular expression of phosphorylated-NF-kB p65 (Ser536; active NF-kB) (145.0+34.1 vs 114.5+30.4 AU). Expression of phosphorylated-p38-MAPK (15.4+5.7 vs 9.2+2.5 AU) and active caspase-3 (168.2±65.5 vs 107.8±40.5 AU), markers of cell apoptosis, was higher in cells treated with obesity-related EMVs. Phosphorylated-eNOS (Ser...
Source: American Journal of Physiology. Endocrinology and Metabolism - Category: Physiology Authors: Source Type: research