< i > Bothrops < /i > venom-induced hemostasis disorders in the rat: Between Scylla and Charybdis

by S ébastien Larréché, Lucie Chevillard, Georges Jourdi, Simon Mathé, Aurélie Servonnet, Bérangère S. Joly, Virginie Siguret, Jean-Philippe Chippaux, Bruno Mégarbane Hemostasis impairment represents the most threatening consequence of Viperidae envenoming, notably withBothrops genus. In the French departments of America,B.atrox envenomation in French Guiana may lead to bleeding whileB.lanceolatus envenomation in Martinique to thrombosis. Bleeding related toB.atrox envenomation is attributed to vascular damage mediated by venom metalloproteinases and blood uncoagulable state resulting from thrombocytopenia and consumptive coagulopathy. Thrombosis related toB.lanceolatus envenomation are poorly understood. We aimed to compare the effects ofB.atrox andB.lanceolatus venoms in the rat to identify the determinants of the hemorrhagicversus thrombotic complications. Viscoelastometry (ROTEM), platelet count, plasma fibrinogen, thrombin generation assay, fibrinography, endothelial (von Willebrand factor, ADAMTS13 activity, ICAM-1, and soluble E-selectin), and inflammatory biomarkers (IL-1 β, IL-6, TNF-α, MCP-1, and PAI-1) were determined in blood samples obtained at H3, H6, and H24 after the subcutaneous venomversus saline injection. In comparison to the control, initial fibrinogen consumption was observed with the two venoms while thrombocytopenia and reduction in the clot amplitude only withB.atrox venom. Moreover, we showed an increase in thrombin generation at H3 with t...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research