Investigation of Antibacterial Activity and Wound Healing Promotion Properties Induced by Bromelain-Loaded Silver Nanoparticles

AbstractWith the demand for better chronic wound care, the clinical problem of managing wound infections has already reached a critical level. Therefore, the development of novel antibiotic-free wound dressings is essential for wound care. The proteolytic enzyme bromelain, silver nanoparticles (AgNPs), and bromelain loaded on silver nanoparticles (Br-AgNPs) were assessed for their impact on wound healing in the albino mice. The formulated nanoparticles were characterized using different techniques, i.e., ultraviolet (UV), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analysis. The antibacterial and fungal activities of the formulated nanoparticles were also investigated. After 5  days of treatment, the wound healing progression and wound size reduction rate were monitored. In addition, serum and skin tissue samples were collected to assess the changes in inflammation levels and observe the histological changes. The results indicated a spherical shape and regular distributi on of the synthesized gold nanoparticles, with an average particle size of 41.33–61.30 nm, where the size was about 39.84 and 82.91 nm after loading bromelain. The Br-AgNPs were the most active antimicrobial agents againstStaphylococcus aureus,Escherichia coli,Klebsiella pneumoniae, andCandida albicans, as compared to the other samples and positive controls. The fibronectin and collagen III levels were significantly increased in the animal groups treated with bromelain and Br-AgNPs (71...
Source: Plasmonics - Category: Biomedical Science Source Type: research