Antibiofilm potential of gallic acid against < em > Klebsiella pneumoniae < /em > and < em > Enterobacter hormaechei < /em > : < em > in-vitro < /em > and < em > in-silico < /em > analysis

This study aimed to assess the anti-biofilm activity of gallic acid (GA) against E. hormaechei and K. pneumoniae. The results of biofilm quantification assays demonstrated that GA exhibited significant antibiofilm activity against E. hormaechei and K. pneumoniae at concentrations of 4 mg mL-1, 2 mg mL-1, 1 mg mL-1, and 0.5 mg mL-1. Similarly, GA exhibited a dose-dependent reduction in violacein production, a QS-regulated purple pigment, indicating its ability to suppress violacein production and disrupt QS mechanisms in Chromobacterium violaceum. Additionally, computational tools were utilized to identify the potential target involved in the biofilm formation pathway. The computational analysis further indicated the strong binding affinity of GA to essential biofilm regulators, MrkH and LuxS, suggesting its potential in targeting the c-di-GMP and quorum sensing (QS) pathways to hinder biofilm formation in K. pneumoniae. These compelling findings strongly advocate GA as a promising drug candidate against biofilm-associated infections caused by E. hormaechei and K. pneumoniae.PMID:37975308 | DOI:10.1080/08927014.2023.2279996

Source: Biofouling - November 17, 2023 Category: Molecular Biology Authors: Pooja P Rajan Praveen Kumar Minsa Mini Devi Jayakumar Parvathi Vaikkathillam Sneha Asha Aparna Mohan Manjusree S Source Type: research