The structural mechanism of dimeric DONSON in replicative helicase activation

Cvetkovic, Passaretti, et  al. determine the cryo-EM structure of a double CMG bound by dimeric DONSON, explaining how the CMG helicase is assembled symmetrically in metazoans. MCM rings become misaligned and poised to cross paths supporting replication origin activation. Disrupting DONSON dimer affects DNA replication, exp laining etiology of primordial dwarfism.

Source: Molecular Cell - October 10, 2023 Category: Cytology Authors: Milos A. Cvetkovic, Paolo Passaretti, Agata Butryn, Alicja Reynolds-Winczura, Georgia Kingsley, Aggeliki Skagia, Cyntia Fernandez-Cuesta, Divyasree Poovathumkadavil, Roger George, Anoop S. Chauhan, Satpal S. Jhujh, Grant S. Stewart, Agnieszka Gambus, Ales Tags: Article Source Type: research