Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii

Nature Protocols, Published online: 14 November 2023; doi:10.1038/s41596-023-00910-yThis protocol enables genetic manipulation of nuclear and cytoplasmic giant viruses and their host, Acanthomoeba castellanii, by using either CRISPR–Cas9 or homologous recombination.

Source: Nature Protocols - November 14, 2023 Category: Biomedical Science Authors: Nadege Philippe Avi Shukla Chantal Abergel Hugo Bisio Source Type: research