B < sub > 12 < /sub > -dependent radical SAM enzymes: Ever expanding structural and mechanistic diversity

Curr Opin Struct Biol. 2023 Nov 4;83:102725. doi: 10.1016/j.sbi.2023.102725. Online ahead of print.ABSTRACTIn the last decade, B12-dependent radical SAM enzymes have emerged as central biocatalysts in the biosynthesis of a myriad of natural products. Notably, these enzymes have been shown to catalyze carbon-carbon bond formation on unactivated carbon atoms leading to unusual methylations. Recently, structural studies have revealed unprecedented insights into the complex chemistry catalyzed by these enzymes. In this review, we cover recent advances in our understanding of B12-dependent radical SAM enzymes from a mechanistic and structural perspective. We discuss the unanticipated diversity of these enzymes which suggests evolutionary links between various biosynthetic and metabolic pathways from antibiotic to RiPP and methane biosynthesis.PMID:37931378 | DOI:10.1016/j.sbi.2023.102725

Source: Current Opinion in Structural Biology - November 6, 2023 Category: Biology Authors: Alhosna Benjdia Olivier Berteau Source Type: research