Engineering status of protein for improving microbial cell factories

Biotechnol Adv. 2023 Nov 6:108282. doi: 10.1016/j.biotechadv.2023.108282. Online ahead of print.ABSTRACTWith the development of metabolic engineering and synthetic biology, microbial cell factories (MCFs) have provided an efficient and sustainable method to synthesize a series of chemicals from renewable feedstocks. However, the efficiency of MCFs is usually limited by the inappropriate status of protein. Thus, engineering status of protein is essential to achieve efficient bioproduction with high titer, yield and productivity. In this review, we summarize the engineering strategies for metabolic protein status, including protein engineering for boosting microbial catalytic efficiency, protein modification for regulating microbial metabolic capacity, and protein assembly for enhancing microbial synthetic capacity. Finally, we highlight future challenges and prospects of improving microbial cell factories by engineering status of protein.PMID:37939975 | DOI:10.1016/j.biotechadv.2023.108282

Source: Biotechnology Advances - November 8, 2023 Category: Biotechnology Authors: Pei Zhou Cong Gao Wei Song Wanqing Wei Jing Wu Liming Liu Xiulai Chen Source Type: research