Low ‐cost, 3D printed irradiation system for in vitro photodynamic therapy experiments
This report addresses the need for improved in vitro photodynamic therapy (PDT) illumination systems, focusing on a practical setup for multiwell plates. It aims to enhance the quality of PDT research on cell cultures in a user-friendly and reproducible manner. Current research lacks cost-effective, automated setups for PDT on cell cultures. W e introduce an economical setup, using a commercial LED lamp with a cooling unit in a 3D-printed enclosure for multiwell irradiation. The setup is versatile, accommodating various wavelengths and illumination field adjustments. The study evaluates its performance by measuring light intensity, unifor mity, photon distribution, and temperature changes during irradiation. AbstractThe development of a suitable irradiation setup is essential for in vitro experiments in photodynamic therapy (PDT). While various irradiation systems have been developed for PDT, only a few offer practical and high-quality setups for precise and reproducible results in cell culture experiments. This report introduces a cost-effective illumination setup designed f or in vitro photodynamic treatments. The setup consists of a commercially available light-emitting diode (LED) lamp, a cooling unit, and a specially designed 3D-printed enclosure to accommodate a multiwell plate insert. The LED lamp is versatile, supporting various irradiation wavelengths and adjus table illumination fields, ensuring consistent and reliable performance. The study evaluates the setup...
Source: Photochemistry and Photobiology - Category: Science Authors: Chris Acquah,
Zachary Pabis,
Sourav Kanti Seth,
Liraz Levi,
Carlos E. Crespo ‐Hernández Tags: METHODS ARTICLE Source Type: research