DRG2 in macrophages is crucial for initial inflammatory response and protection against Listeria monocytogenes infection

Clin Immunol. 2023 Oct 31:109819. doi: 10.1016/j.clim.2023.109819. Online ahead of print.ABSTRACTInnate immune response is critical for the control of Listeria monocytogenes infection. Here, we identified developmentally regulated GTP-binding protein 2 (DRG2) in macrophages as a major regulator of the innate immune response against L. monocytogenes infection. Both whole-body DRG2 knockout (KO) mice and macrophage-specific DRG2 KO mice had low levels of IL-6 during early infection and increased susceptibility to L. monocytogenes infection. Following an initial impaired inflammatory response of macrophages upon i.p. L. monocytogenes infection, DRG2-/- mice showed delayed recruitment of neutrophils and monocytes into the peritoneal cavity, which led to elevated bacterial burden, inflammatory cytokine production at a late infection time point, and liver micro-abscesses. DRG2 deficiency decreased the transcriptional activity of NF-κB and impaired the inflammatory response of both bone marrow-derived and peritoneal macrophages upon L. monocytogenes stimulation. Our findings reveal that DRG2 in macrophages is critical for the initial inflammatory response and protection against L. monocytogenes infection.PMID:37918467 | DOI:10.1016/j.clim.2023.109819

Source: Clinical Immunology - November 2, 2023 Category: Allergy & Immunology Authors: Unn Hwa Lee Sang Jin Park Seong A Ju Sang Chul Lee Byung Sam Kim Byungyong Ahn Jawoon Yi Jihwan Park Young-Wook Won In Seob Han Byung Ju Lee Wha Ja Cho Jeong Woo Park Source Type: research