38. Assessment of TRG and TRB clonality by NGS of dermatologic specimens is impacted by biopsy type, DNA and amplicon sizes

Molecular testing to detect clonal T-cell populations is commonly applied to dermatologic specimens to support cutaneous T-cell lymphoma diagnosis. With clinical implementation of TRG and TRB clonality by NGS using Lymphotrack (Invivoscribe) sequenced on the Miseq (Illumina), increased specimen numbers have been submitted for testing. For 66 specimens (50 patients) received to date, high insufficiency rates were found for TRG (30%) and TRB (56%). Thus, to improve testing efficiency and effect better specimen triage, we examined tissue features, DNA size by fragment analysis, library concentration, reads and overall call.
Source: Cancer Genetics and Cytogenetics - Category: Genetics & Stem Cells Authors: Source Type: research