84. Challenges in the clinical implementation of the large next-generation sequencing oncomine comprehensive plus assay

Technical barriers, panel complexity and bioinformatics are major challenges for the clinical implementation of large NGS panels. For optimization of the 500+ gene Oncomine comprehensive plus targeted amplicon based NGS assay to detect SNVs, Indels, CNVs, fusions and TMB, 40ng DNA and RNA from 115 specimens from different tumor types (tumor cellularity ≥10%) were used for library preparation and sequenced on the Ion S5XL sequencer. Ion Reporter 5.18 (IR) and Alissa custom classification trees were optimized for known variants.
Source: Cancer Genetics and Cytogenetics - Category: Genetics & Stem Cells Authors: Source Type: research