Extracts of Trichocline sinuata (Asteraceae) as natural sensitizers in the photodynamic inactivation of Candida albicans

Flora in the east-central region of Argentina may offer a vast array of opportunities to isolate new photoactive molecules or plant extracts, with high potential for use in antimicrobial photodynamic therapy (aPDT) against yeast. For this purpose, the photofungicidal potential ofTricholine sinuata ( “contrayerba”) againstCandida Albicans was determined. The highest photoinactivation againstC. albicans was observed for dicholormethanic extracts (MFC  = 62.5 μg/mL). Furthermore, we determine that photochemical mechanisms dependent on oxygen (type I and type II) and independent of oxygen (photoadduct formation) are involved in the death of these yeasts. AbstractDespite significant progress in the development of phototherapy drugs, it is widely recognized that natural products remain the primary source of new photoactive compounds. Exploring uncharted flora in the east-central region of Argentina may offer a vast array of opportunities to isolate new photoactive molecules or plant extracts with high potential for use in antimicrobial photodynamic therapy (aPDT) againstCandida albicans. To assess the photofungicidal potential ofT. sinuata ( “contrayerba”) againstC. albicans, the extracts underwent spectroscopic and chromatographic analysis, resulting in the identification of furanocoumarin metabolites with similar spectrophotometric properties in all extracts. The extract profiles were created using UHPLC-DAD, and seven furanocoumarins (FCs) were detected. The highe...
Source: Photochemistry and Photobiology - Category: Science Authors: Tags: RESEARCH ARTICLE Source Type: research