Advancement of microfluidic modulation spectroscopy as a highly sensitive protein characterization technique for the detection of small structural changes

This study evaluates and compares the different well plate formats and scan modes of two MMS instruments. The newer Apollo system features a high throughput 96-well plate format and sweep scan mode that allows a 50% reduction in sample volume consumption and measurement time compared to the previous system. By measuring two proteins with drastically different secondary structures, the results demonstrated that the measurements were highly repeatable (>99% repeatability by area of overlap) regardless of the well plate formats or the scan modes. The limit of quantitation (LOQ) for determining structural impurity using the sweep scan mode was 3.2% and significantly better than that of FTIR at 23% from previous studies. This work highlighted the advancement of MMS as a highly sensitive technique to detect small changes of protein structures due to aggregation or misfolding.PMID:37858878 | DOI:10.1016/j.ab.2023.115350

Source: Analytical Biochemistry - October 20, 2023 Category: Biochemistry Authors: Tianhui Maria Ma Richard H Huang Valerie I Collins Mats Wikstr öm Dipanwita Batabyal Source Type: research