An ionic liquid-assisted sample preparation method for sensitive integral-membrane proteome analysis

In this study, we present a combined i-soln-assisted proteomic sample preparation platform (termed pTRUST), which is compatible with starting materials in the sub-microgram range, using our previously reported i-soln-based sample preparation strategy (iBOPs) and an in-StageTip technique. This novel and straightforward approach allows for the rapid solubilization and processing of a variety of IMPs from human samples to support highly sensitive mass spectrometry analysis. We also demonstrated that the performance of this technology surpasses that of conventional methods such as filter-aided sample preparation methods, FASP and i-FASP. The convenience and availability of pTRUST technology using the IL system have great potential for proteomic identification and characterization of novel drug targets and disease biology in research and clinical settings.PMID:37852348 | DOI:10.1016/j.ab.2023.115349

Source: Analytical Biochemistry - October 18, 2023 Category: Biochemistry Authors: Masato Taoka Kota Kamei Akiko Kashima Yuko Nobe Takahiro Takekiyo Takamasa Uekita Tohru Ichimura Source Type: research