Metabolism of serine/glycine lipids by human gingival cells in culture

This study revealed that treatment of HGF or macrophage (THP1) cells with lipid (L) 654 resulted in breakdown to L342 and s ubsequent release into culture medium. However, L654 was converted only to L567 in gingival keratinocytes. By contrast, L1256 was converted to L654 by fibroblasts and macrophages but no further hydrolysis or release into medium was observed. Gingival keratinocytes showed no hydrolysis of L1256 to sm aller lipid products but because L1256 was not recovered in these cells, it is not clear what hydrolysis products are produced from L1256. Although primary cultures of gingival fibroblasts and macrophages are capable of hydrolyzing specific serine/glycine lipids, prior analysis of lipid extracts fro m diseased gingival tissues revealed significantly elevated levels of L1256 in diseased tissues. These results suggest that the hydrolysis of bacterial lipids in gingival tissues may reduce the levels of specific lipids, but the hydrolysis of L1256 is not sufficiently rapid to prevent significant ac cumulation at periodontal disease sites.

Source: Molecular Oral Microbiology - October 18, 2023 Category: Microbiology Authors: Tyler M. Guido, Samuel D. Ratcliffe, Amanda Rahmlow, Matthew A. Zambrello, Anthony A. Provates, Robert B. Clark, Michael B. Smith, Frank C. Nichols Tags: ORIGINAL ARTICLE Source Type: research