Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages

Innate Immun. 2023 Oct 13:17534259231205993. doi: 10.1177/17534259231205993. Online ahead of print.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profiles or colony forming units and time to death following Salmonella infection as compared to controls. Additionally, p65fl/fl/LysMCre mice, especially females, display splenomegaly, but no other obvious physical or behavioral differences as compared to control animals. As bone marrow-derived macrophages from this transgenic model are almost completely devoid of canonical nuclear factor-kappaB pathway member p65, this model has the potential for being very useful in investigating bone marrow-derived macrophage NF-kappaB signaling in diverse biological and biomedical studies.PMID:37828842 | DOI:10.1177/17534259231205993
Source: Innate Immunity - Category: Allergy & Immunology Authors: Source Type: research