Impact of cell-debris and room-temperature storage on urine circulating tumor DNA from hepatocellular carcinoma

This study evaluated the impact of cell debris and 7-day room temperature (RT) storage on the quality and yield of transrenal DNA. Archived urine specimens collected from five hepatocellular carcinoma (HCC) patients and two pregnant women (PG) carrying male fetuses were used to assess the impact of cell-debris on urine cell-free DNA (ucfDNA) isolation as measured by quantitative PCR (qPCR) for Y-chromosome (Y-Chr) DNA, or HCC-associated mutation and methylation markers, and by capillary electrophoresis.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Regular Article Source Type: research