Application of sample displacement batch chromatography for fractionation of proteoforms

In this study, we demonstrate that sample displacement batch chromatography (SDBC) is an easy-to-handle, economical, and efficient method for fractionating proteoforms. As a model sample a commercial ovalbumin fraction was used, containing many ovalbumin proteoforms. The most promising parameters for the SDBC were determined by a screening approach and applied for a 10-segment fractionation of ovalbumin with cation exchange chromatography resins. Mass spectrometry of intact proteoforms was used for characterizing the SDBC fractionation process. By SDBC, a significant separation of different proteoforms was obtained.

Source: Proteomics - September 27, 2023 Category: Biochemistry Authors: Siti Nurul Hidayah, Ali Biabani, Manasi Gaikwad, Paula Nissen, Hannah Vo ß, Maria Riedner, Hartmut Schlüter, Bente Siebels Tags: RESEARCH ARTICLE Source Type: research