GSE243401 A Serum- and Feeder-Free System to Generate CD4 and Regulatory T Cells from Human iPSCs

Contributors : Simona Zompi ; Helen Fong ; Matthew Mendel ; John Jascur ; Laeya Najmi ; Ken Kim ; Garrett Lew ; Swetha Garimalla ; Suruchi Schock ; Jing Hu ; Andres Villegas ; Anthony Conway ; Jason FontenotSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWhile feeder-free generation of clinical grade iPSC-derived CD8 T cells has been achieved, differentiation of iPSC-derived CD4sp and regulatory T cells requires mouse stromal cells in an artificial thymic organoid. We developed a serum- and feeder-free differentiation process suitable for large-scale production and used scRNAseq to study the cell identity of the iPSC-derived cells and compared their transcriptional profile to primary human cells.

Source: GEO: Gene Expression Omnibus - September 21, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research