Genome-wide identification, evolution and expression of TGF- β signaling pathway members in mandarin fish (Siniperca chuatsi)

Int J Biol Macromol. 2023 Sep 16:126949. doi: 10.1016/j.ijbiomac.2023.126949. Online ahead of print.ABSTRACTMembers of the transforming growth factor β (TGF-β) signaling pathway regulate diverse cellular biological processes in embryonic and tissue development. We took mandarin fish (Siniperca chuatsi) as the research object to identify all members of the TGF-β signaling pathway, measure their expression pattern in the key period post hatching, and further explore their possible role in the process of sex regulation. Herein, we identified eighty-three TGF-β signaling pathway members and located them on chromosomes based on the genome of mandarin fish. TGF-β signaling pathway members were highly conserved since each TGF-β subfamily clustered with orthologs from other species. Transcriptome analysis, qRT-PCR and in situ hybridization demonstrated that most mandarin fish TGF-β signaling pathway members presented stage-specific and/or sex-dimorphic expression during gonadal development, and different members of the TGF-β signaling pathway participated in different stages of gonadal development. Taken together, our results provide new insight into the role of TGF-β signaling pathway members in the sex regulation of mandarin fish.PMID:37722635 | DOI:10.1016/j.ijbiomac.2023.126949

Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Shiyan Liu Chong Han Jingjun Huang Meihui Li Jiayu Yang Guifeng Li Haoran Lin Shuisheng Li Yong Zhang Source Type: research