Structure-based mining of a chitosanase with distinctive degradation mode and product specificity

This study succeeded in discovering a chitosanase with distinctive degradation modes, which could facilitate the mechanistic understanding of chitosanases, further empowering the production of chitosan derivates with specific DP. KEY POINTS: • Structural docking and evolutionary analysis guide to mining the chitosanase. • The endo-chitosanase exhibits a unique GlcN-producing cleavage pattern. • The cleavage direction of chitosanase to produce GlcN was identified.PMID:37713113 | DOI:10.1007/s00253-023-12741-8
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Source Type: research