Viruses, Vol. 15, Pages 1931: Kinetics of TTV Loads in Peripheral Blood Mononuclear Cells of Early Treated Acute HIV Infections

In this study, TTV replication was evaluated in 21 people with acute HIV infection (AHI) and immune reconstitution following antiretroviral therapy (ART). PBMC-associated TTV and HIV-1 DNA, as well as plasma HIV-1 RNA, were measured by real-time PCR. CD4 and CD8 differentiation, activation, exhaustion, and senescence phenotypes were analyzed by flow cytometry. Thirteen healthy donors (HD) and twenty-eight chronically infected HIV individuals (CHI), late presenters at diagnosis, were included as control groups. TTV replication in AHI seems to be controlled by the immune system being higher than in HD and lower than in CHI. During ART, a transient increase in TTV DNA levels was associated with a significant perturbation of activation and senescence markers on CD8 T cells. TTV loads were positively correlated with the expansion of CD8 effector memory and CD57+ cells. Our results shed light on the kinetics of TTV replication in the context of HIV acute infection and confirm that the virus replication is strongly regulated by the modulation of the immune system.

Source: Viruses - September 15, 2023 Category: Virology Authors: Isabella Abbate Gabriella Rozera Eleonora Cimini Fabrizio Carletti Eleonora Tartaglia Marika Rubino Silvia Pittalis Rozenn Esvan Roberta Gagliardini Annalisa Mondi Valentina Mazzotta Marta Camici Enrico Girardi Francesco Vaia Vincenzo Puro Andrea Antinori Tags: Article Source Type: research