PYK-SubstitutionOME: an integrated database containing allosteric coupling, ligand affinity and mutational, structural, pathological, bioinformatic and computational information about pyruvate kinase isozymes

We report here a database derived from mutational, biochemical, bioinformatic, structural, pathological and computational studies of a highly studied protein family —pyruvate kinase (PYK). A centerpiece of this database is the biochemical characterization—including quantitative evaluation of allosteric regulation—of the changes that accompany substitutions at positions that sample the full conservation range observed in the PYK family. We have used these data to facilitate critical advances in the foundational studies of allosteric regulation and protein evolution and as rigorous benchmarks for testing protein predictions. We trust that the collected dataset will be useful for the broader scientific community in the further development of prediction algorithms.Database URLhttps://github.com/djparente/PYK-DB
Source: Database : The Journal of Biological Databases and Curation - Category: Databases & Libraries Source Type: research