Criteria analysis for kinetics curves of initiated blood serum chemiluminescence

Acta Biochim Pol. 2023 Sep 7. doi: 10.18388/abp.2020_6807. Online ahead of print.ABSTRACTThe chemiluminescence (CL) methods unlike the other methods of determining free radicals (FR) allow investigating the kinetics of the derivation and recombination of radicals/antioxidants, and thus the development and attenuation of the process/processes after excitation in time. However, these methods are of limited application because the knowledge of the explored parameters is insufficient (maximum intensity and integrated area under the kinetics plot). The kinetics is studied by the CL methods and a new parameter (IR-criterion) of analysis of damping of the initiated CL dynamics has been introduced. The IR-criterion parameter: identifies the relationship between the rates of initiation and recombination of peroxide radicals in blood-serum samples; allows the full straightening of the CL curves; provides new information in the considered pathological processes; can serve as an additional universal characteristic of FR activity of blood serum in pathological processes.PMID:37677090 | DOI:10.18388/abp.2020_6807

Source: Acta Biochim Pol - September 7, 2023 Category: Biochemistry Authors: Iryna Oliynyk Source Type: research