Transcription-coupled nucleosome assembly

Trends Biochem Sci. 2023 Aug 30:S0968-0004(23)00206-2. doi: 10.1016/j.tibs.2023.08.003. Online ahead of print.ABSTRACTEukaryotic transcription occurs on chromatin, where RNA polymerase II encounters nucleosomes during elongation. These nucleosomes must unravel for the DNA to enter the active site. However, in most transcribed genes, nucleosomes remain intact due to transcription-coupled chromatin assembly mechanisms. These mechanisms primarily involve the local reassembly of displaced nucleosomes to prevent (epi)genomic instability and the emergence of cryptic transcription. As a fail-safe mechanism, cells can assemble nucleosomes de novo, particularly in highly transcribed genes, but this may result in the loss of epigenetic information. This review examines transcription-coupled chromatin assembly, with an emphasis on studies in yeast and recent structural studies. These studies shed light on how elongation factors and histone chaperones coordinate to enable nucleosome recycling during transcription.PMID:37657993 | DOI:10.1016/j.tibs.2023.08.003

Source: Trends in Biochemical Sciences - September 1, 2023 Category: Biochemistry Authors: Fran çois Robert C élia Jeronimo Source Type: research