Genetic rearrangements in Pseudomonas amygdali pathovar aesculi shape coronatine plasmids

In this study, we have sequenced and analyzed the complete genome sequences of four Pseudomonas amygdali pv. aesculi (Pae), which infect European horse chestnut trees (Aesculus hippocastanum) and belong to phylogroup 3 of the P. syringae species complex. The four investigated genomes contain six groups of plasmids that all encode pathogenicity factors. Effector genes were found to be mostly associated with insertion sequence elements, suggesting that virulence genes are generally mobilized and potentially undergo horizontal gene transfer after transfer to a conjugative plasmid. We show that the biosynthetic gene cluster encoding the phytotoxin coronatine was recently transferred from a chromosomal location to a mobilizable plasmid that subsequently formed a co-integrate with a conjugative plasmid.PMID:37541538 | DOI:10.1016/j.meegid.2023.105486

Source: Infection, Genetics and Evolution - August 4, 2023 Category: Genetics & Stem Cells Authors: Tue Kj ærgaard Nielsen Caroline S Winther-Have Iben Margrete Thomsen Robert W Jackson Mojgan Rabiey Rosanna Catherine Hennessy Frederik Bak Witold Kot Mette Haubjerg Nicolaisen Alexander Byth Carstens Lars Hestbjerg Hansen Source Type: research