Protein-protein interaction-mediated regulation of lysine biosynthesis of Thermus thermophilus through the function-unknown protein LysV

J Gen Appl Microbiol. 2023 Jun 26. doi: 10.2323/jgam.2023.06.003. Online ahead of print.ABSTRACTT. thermophilus biosynthesizes lysine via α-aminoadipate as an intermediate using the amino-group carrier protein, LysW, to transfer the attached α-aminoadipate and its derivatives to biosynthetic enzymes. A gene named lysV, which encodes a hypothetical protein similar to LysW, is present in the lysine biosynthetic gene cluster. Although the knockout of lysV did not affect lysine auxotrophy, lysV homologs are conserved in the lysine biosynthetic gene clusters of microorganisms belonging to the phylum Deinococcus-Thermus, suggesting a functional role for LysV in lysine biosynthesis. Pulldown assays and crosslinking experiments detected interactions between LysV and all of the biosynthetic enzymes requiring LysW for reactions, and the activities of most of all these enzymes were affected by LysV. These results suggest that LysV modulates the lysine biosynthesis through protein-protein interactions.PMID:37357393 | DOI:10.2323/jgam.2023.06.003

Source: Journal of General and Applied Microbiology - June 26, 2023 Category: Microbiology Authors: Yutaro Morita Ayako Yoshida Siyan Ye Takeo Tomita Minoru Yoshida Saori Kosono Makoto Nishiyama Source Type: research