Molecules, Vol. 28, Pages 4911: Implications of Pharmacokinetic Potentials of Pioglitazone Enantiomers in Rat Plasma Mediated through Glucose Uptake Assay

Molecules, Vol. 28, Pages 4911: Implications of Pharmacokinetic Potentials of Pioglitazone Enantiomers in Rat Plasma Mediated through Glucose Uptake Assay
Molecules doi: 10.3390/molecules28134911
Authors:
Tatineni Spandana
Veera Venkata Nishanth Goli
Mohamed Rahamathulla
Sirajunisa Talath
Riyaz Ali M. Osmani
Mohammed Muqtader Ahmed
Syeda Ayesha Farhana
Shalam Mohamed Hussain
Bannimath Gurupadayya

Pioglitazone, a PPAR-gamma activator used to diagnose hyperglycemia, was studied for its stereoselective deposition and active enantiomers in female albino Wistar rats. In accordance with USFDA recommendations, a bioanalytical technique was employed to assess the segregation of pioglitazone enantiomers in rat plasma with glimepiride as an internal standard. A Phenomenox i-Amylose-3 column (150 mm × 4.6 mm) of 5 µm was used for high-performance liquid chromatography (HPLC) with a mobile phase of 10 mM ammonium acetate buffer in Millipore water and acetonitrile in 60:40 (v/v) admixture with column temperature 35 °C, wavelength 265 nm, and flow rate 0.6 mL/min, respectively. Pioglitazone-S, Pioglitazone-R, and the internal standard had retention times of 3.1, 7.4, and 1.7 min, respectively. The study found that within-run and between-run precision ranged from 0.1606–0.9889% for Pioglitazone-R and from 0.2080–0.7919% for Pioglitazone-S, while the accuracy ranged from 99.86 to 100.36% for Pioglitaz...

Source: Molecules - June 22, 2023 Category: Chemistry Authors: Tatineni Spandana Veera Venkata Nishanth Goli Mohamed Rahamathulla Sirajunisa Talath Riyaz Ali M. Osmani Mohammed Muqtader Ahmed Syeda Ayesha Farhana Shalam Mohamed Hussain Bannimath Gurupadayya Tags: Article Source Type: research