Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing

Single-cell RNA sequencing (scRNA-seq) has advanced our understanding of skin biology, but its utility is restricted by the requirement of fresh samples, inadequate dissociation-induced cell loss or death, and activation during tissue digestion. Single-nucleus RNA sequencing (snRNA-seq) can use frozen, hard-to-dissociate materials, which might be a promising method to circumvent the limitations of scRNA-seq for the skin tissue.

Source: Journal of Dermatological Science - June 19, 2023 Category: Dermatology Authors: Ronghui Zhu, Xiaoyu Pan, Shangshang Wang, Zhuoqiong Qiu, Chaoying Gu, Xu Yao, Wei Li Source Type: research