Development of Scaffold-Free Three-Dimensional Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis

Organoids are novel in vitro models to study intercellular cross talk between the different types of cells in the disease pathophysiology. To better understand the underlying mechanisms driving the progression of primary sclerosing cholangitis (PSC), we developed scaffold-free multicellular three-dimensional cholangiocy te organoids (3D-CHOs) using primary liver cells derived from normal patients and patients with PSC. Human liver samples from healthy donors and patients with PSC were used to isolate primary cholangiocytes [epithelial cell adhesion molecule (EpCam)+/ cytokeratin-19+], liver endothelial cells (CD31+ ), and hepatic stellate cells (HSCs; CD31−/CD68−/desmin+/vitamin A+).

https://ajp.amjpathol.org/article/S0002-9440(23)00197-9/fulltext?rss=yes

Source: American Journal of Pathology - May 30, 2023 Category: Pathology Authors: Wenjun Zhang, Konstantina Kyritsi, Abdulkadir Isidan, Yujin Park, Ping Li, Arthur A. Cross-Najafi, Kevin Lopez, Lindsey Kennedy, Keisaku Sato, Shannon Glaser, Heather Francis, Gianfranco Alpini, Burcin Ekser Tags: Regular article Source Type: research