Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors

Nature Protocols, Published online: 05 June 2023; doi:10.1038/s41596-023-00837-4Detect-seq (dU-detection enabled by C-to-T transition during sequencing) enables off-target evaluation of programmable cytosine base editors. DNA is chemically labeled, and the editing intermediate dU is enriched for sequencing by biotin pull-down.

Source: Nature Protocols - June 5, 2023 Category: Biomedical Science Authors: Zhixin Lei Haowei Meng Xichen Rao Huanan Zhao Chengqi Yi Source Type: research