Novel Non-Hot Spot Modification in Fks1 of Candida auris Confers Echinocandin Resistance

Antimicrob Agents Chemother. 2023 May 24:e0042323. doi: 10.1128/aac.00423-23. Online ahead of print.ABSTRACTWe determined the echinocandin susceptibility and FKS1 genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel FKS1 mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to 32×; caspofungin, >64×; micafungin, >64×).PMID:37222585 | DOI:10.1128/aac.00423-23

Source: Antimicrobial Agents and Chemotherapy - May 24, 2023 Category: Microbiology Authors: Milena Kordalewska Geselle Cancino-Prado Jo ão Nobrega de Almeida Júnior Igor Brasil Brand ão Renata Tigulini de Souza Peral Arnaldo L Colombo David S Perlin Source Type: research