Development of Scaffold-free 3D Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis

Organoids are novel in vitro models to study intercellular crosstalk between the different types of cells in the disease pathophysiology. To better understand the underlying mechanisms driving the progression of primary sclerosing cholangitis (PSC), we developed scaffold-free multi-cellular 3D cholangiocyte organoids (3D-CHO) using ‘primary’ liver cells derived from normal and PSC patients. Human liver samples from healthy donors and PSC patients were used to isolate ‘primary’ cholangiocytes (EPCAM+/CK-19+), liver endothelial cells (LECs, CD31+), and hepatic stellate cells (HSCs, CD31-/CD68-/ Desmin+/Vitamin A+).

Source: American Journal of Pathology - May 30, 2023 Category: Pathology Authors: Wenjun Zhang, Konstantina Kyritsi, Abdulkadir Isidan, Yujin Park, Ping Li, Arthur A. Cross-Najafi, Kevin Lopez, Lindsey Kennedy, Keisaku Sato, Shannon Glaser, Heather Francis, Gianfranco Alpini, Burcin Ekser Tags: Regular Article Source Type: research