Efficiency of genetic modification in gene ‐knockout sperm‐derived zygotes followed by electroporation of guide RNA targeting the same gene

In conclusion, the combination of fertilization with gene-deficient spermatozoa and gene editing of the same targeted gene region using EP had no beneficial effects on embryo genetic modification, indicating that EP alone is a sufficient tool for genome modification.

Source: Animal Science Journal - May 23, 2023 Category: Zoology Authors: Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Nanaka Torigoe, Bin Liu, Maki Hirata, Megumi Nagahara, Fuminori Tanihara, Takeshige Otoi Tags: TECHNICAL NOTE Source Type: research