Transmissive multifocal laser speckle contrast imaging through thick tissue

In this study, we propose a transmissive multifocal LSCI for depth-resolved blood flow in thick tissue, further extending the transmissive LS CI for tissue thickness up to [math]. The limited-DOF imaging system is applied to the multifocal acquisition, and the depth of the vessel is estimated using a robust visibility parameter [math] in the coherent domain. The accuracy and linearity of depth estimation are tested by Monte Carlo simulati ons. Based on the proposed method, the model of contrast analysis resolving the depth information is established and verified in a phantom experiment. We demonstrated its effectiveness in acquiring depth-resolved vessel structures and flow dynamics in in vivo imaging of chick embryos.
Source: Journal of Innovative Optical Health Sciences - Category: Biomedical Science Authors: Source Type: research