Sensors, Vol. 23, Pages 4396: Electrochemical Detection of ompA Gene of C. sakazakii Based on Glucose-Oxidase-Mimicking Nanotags of Gold-Nanoparticles-Doped Copper Metal & ndash;Organic Frameworks

Sensors, Vol. 23, Pages 4396: Electrochemical Detection of ompA Gene of C. sakazakii Based on Glucose-Oxidase-Mimicking Nanotags of Gold-Nanoparticles-Doped Copper Metal–Organic Frameworks Sensors doi: 10.3390/s23094396 Authors: Hongyan Zhang Guiqing Xu Yuming Chen Xu Li Shaopeng Wang Feihao Jiang Pengyang Zhan Chuanfu Lu Xiaodong Cao Yongkang Ye Yunlai Tao The present work developed an electrochemical genosensor for the detection of virulence outer membrane protein A (ompA, tDNA) gene of Cronobacter sakazakii (C. sakazakii) by exploiting the excellent glucose-oxidase-mimicking activity of copper metal–organic frameworks (Cu-MOF) doped with gold nanoparticle (AuNPs). The signal nanotags of signal probes (sDNA) that biofunctionalized AuNPs@Cu-MOF (sDNA-AuNPs@Cu-MOF) were designed using an Au-S bond. The biosensor was prepared by immobilization capture probes (cDNA) onto an electrodeposited AuNPs-modified glassy carbon electrode (GCE). AuNPs@Cu-MOF was introduced onto the surface of the GCE via a hybridization reaction between cDNA and tDNA, as well as tDNA and sDNA. Due to the enhanced oxidase-mimicking activity of AuNPs@Cu-MOF to glucose, the biosensor gave a linear range of 1.0 × 10−15 to 1.0 × 10−9 mol L−1 to tDNA with a detection limit (LOD) of 0.42 fmol L−1 under optimized conditions using differential pulse voltammetry measurement (DPV)....
Source: Sensors - Category: Biotechnology Authors: Tags: Article Source Type: research