TaUAM3, a UDP ‐Ara mutases protein, positively regulates wheat resistance to the stripe rust fungus

In this study, we dissected the role of theUAM family member,UAM3, during the interaction between wheat and the stripe rust fungus,Puccinia striiformis f. sp.tritici (Pst), and in response to treatment with salicylic acid (SA). RNA interference (RNAi)-based stable silencing ofTaUAM3 resulted in decreased resistance toPst fungus. In addition, CRISPR-mediated genome editing (GE) ofTaUAM3 enhanced the susceptibility of wheat toPst or compromised disease resistance accompanied by increased fungal growth and decreased H2O2 production in plant tissues. Moreover, the transcript levels of pathogenesis-related (PR) genes and reactive oxygen species (ROS)-generating genes were down-regulated in both the RNAi-silenced and CRISPR-edited plants, while the ROS-scavenging gene,TaCAT3, was up-regulated. Therefore,TaUAM3 positively regulates the resistance of wheat toPst.

Source: Food and Energy Security - March 18, 2023 Category: Food Science Authors: Xueling Huang, Xingxuan Bai, Chaowei Qian, Shuai Liu, Farhan Goher, Fuxin He, Guosen Zhao, Guoliang Pei, Hua Zhao, Jianfeng Wang, Zhensheng Kang, Jun Guo Tags: ORIGINAL ARTICLE Source Type: research