Enterococcus moraviensis EMo 1-1Nik of horse origin:characteristics and potential bacteriocin-producing strain

AbstractNowadays, developed more precisious identification techniques have allowed to validate newer enterococcal species. Among them, the speciesEnterococcus moraviensis was also validated, at first from surface waters. However, in this study, characteristics and potential to bacteriocin production by the strainE. moraviensis EMo 1-1Nik isolated from buccal mucosa of Slovak warm-blood horse breed has been studied. BLASTn analysis allotted this strain to the speciesE. moraviensis with percentage identity BLASTn 16S rRNA sequence in the strain up to 100% (99.93% similarity withE. moraviensis NR113937.1). The strain EMo 1-1Nik has been provided with GenBank accession number MW326085. It is hemolysis-negative ( γ-hemolysis), deoxyribonuclease-negative and gelatinase-negative; absent of virulence factor genes, low-grade biofilm-positive (0.133 ± 0.36), mostly susceptible to tested antibiotics. Moreover, 60% of EMo1-1Nik colonies were found as bacteriocin-producing against the principal indicatorEnterococcus avium EA5. The concentrated substance (CS, pH 4.5) of EMo1-1Nik showed the inhibitory activity against EA5 strain (800 AU/mL); CSs with pH 6.3 and 7.3 reached inhibitory activity 100 AU/mL against EA5 strain. CS was thermo-stable and it does not lost activity after enzymes treatment. Oppositelly, EMo 1-1Nik was susceptible to Mundticin EM 41/3 (800 AU/mL) produced by horse fecal strainE. mundtii EM 41/3 and enterocins (up to 51  200 AU/mL). In spite of the preliminary r...
Source: Veterinary Research Communications - Category: Veterinary Research Source Type: research