Quantitative analyses of interactions between SpoVG and RNA/DNA

Biochem Biophys Res Commun. 2023 Feb 27;654:40-46. doi: 10.1016/j.bbrc.2023.02.044. Online ahead of print.ABSTRACTThe Borrelia burgdorferi SpoVG protein has previously been found to be a DNA- and RNA-binding protein. To aid in the elucidation of ligand motifs, affinities for numerous RNAs, ssDNAs, and dsDNAs were measured and compared. The loci used in the study were spoVG, glpFKD, erpAB, bb0242, flaB, and ospAB, with particular focus on the untranslated 5' portion of the mRNAs. Performing binding and competition assays yielded that the 5' end of spoVG mRNA had the highest affinity while the lowest observed affinity was to the 5' end of flaB mRNA. Mutagenesis studies of spoVG RNA and ssDNA sequences suggested that the formation of SpoVG-nucleic acid complexes are not entirely dependent on either sequence or structure. Additionally, exchanging uracil for thymine in ssDNAs did not affect protein-nucleic acid complex formation.PMID:36889033 | DOI:10.1016/j.bbrc.2023.02.044

Source: Biochemical and Biophysical Research communications - March 8, 2023 Category: Biochemistry Authors: Timothy C Saylor Christina R Savage Andrew C Krusenstjerna Nerina Jusufovic Wolfram R Z ückert Catherine A Brissette Md Motaleb Paula J Schlax Brian Stevenson Source Type: research