Rapid Lineage Assignment of SARS-CoV-2 Cases Through Automated Library Preparation, Sequencing and Bioinformatic Analysis

The COVID-19 pandemic has provided a stage to illustrate that there is considerable value in obtaining rapid, whole genomic information about pathogens. Herein we describe the utility of a commercially available, automated SARS-CoV-2 library preparation, genomic sequencing and a bioinformatic analysis pipeline to provide rapid, near- “real-time” SARS-CoV-2 variant description. We evaluated the turnaround time, accuracy and other quality parameters obtained from Clear Labs Dx automated sequencing instrumentation from analysis of continuous clinical samples from January 1, 2021 to October 6, 2021.

Source: Journal of Molecular Diagnostics - February 6, 2023 Category: Pathology Authors: Andrew J. Gorzalski, Heather Kerwin, Subhash Verma, David C. Hess, Joel Sevinsky, Kevin Libuit, Irina Vlasova-St. Louis, Danielle Siao, Lauren Siao, Diego Bunuel, Stephanie Van Hooser, Mark W. Pandori Tags: Technical Advance Source Type: research