Age-associated modifications of intestinal permeability and innate immunity in human small intestine

The physical and immunological properties of the human intestinal epithelial barrier in ageing are largely unknown. Ileal biopsies from young (7-12 years), adult (20-40y) and ageing (67-77y) individuals not showing symptoms of gastrointestinal pathologies were used to assess levels of inflammatory cytokines, barrier integrity, and cytokine production in response to microbial challenges. Increased expression of IL-6, but not IFNg, TNF-a and IL-1b was observed during ageing; further analysis showed that CD11c+ dendritic cells (DCs) are one of the major sources of IL-6 in the ageing gut and expressed higher levels of CD40. Up-regulated production of IL-6 was accompanied by increased expression of Claudin-2 leading to reduced transepithelial electric resistance (TEER); TEER could be restored in in vitro and ex-vivo cultures by neutralizing anti-IL-6 antibody. In contrast, expression of Zonula occludens-1, Occludin and Junctional-Adhesion Molecule-A1 did not vary with age and overall permeability to macromolecules was not affected. Finally, cytokine production to different microbial stimuli was assessed in a polarized in vitro organ culture. IL-8 production in response to flagellin declined progressively with age although the expression and distribution of TLR-5 on intestinal epithelial cells remained unchanged. Also, flagellin-induced production of IL-6 was less pronounced in ageing. In contrast, TNF-a production in response to probiotics (VSL#3) did not decline with age; however...
Source: Clinical Science - Category: Biomedical Science Authors: Source Type: research

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CONCLUSION: In the outpatient clinic specializing in oral anticoagulation, patients add more specific beliefs about the interference of vitamin K-rich diet on coagulation, when compared to those in the general cardiology outpatient clinic. PMID: 31618372 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
CONCLUSION: The Tracer Methodology is used for evaluating and improving the quality of care. PMID: 31618371 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
Publication date: Available online 16 October 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Nicole E. Breuner, Shelby L. Ford, Andrias Hojgaard, Lynn M. Osikowicz, Christina M. Parise, Maria F. Rosales Rizzo, Ying Bai, Michael L. Levin, Rebecca J. Eisen, Lars EisenAbstractThe invasive, human-biting Asian longhorned tick, Haemaphysalis longicornis, was detected in New Jersey in the eastern United States in August of 2017 and by November of 2018 this tick had been recorded from 45 counties across 9 states, primarily along the Eastern Seaboard. The establishment of H. longicornis in the United States has raised the ques...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Jinming Wang, Jifei Yang, Shandian Gao, Aihong Liu, Muhammad Rashid, Youquan Li, Zhijie Liu, Junlong Liu, Guangyuan Liu, Jianxun Luo, Guiquan Guan, Hong YinAbstractBovine theileriosis, caused by protozoan parasites of the genus Theileria, presents with various clinical symptoms. In cattle, clinical presentations and outcomes of bovine theileriosis are closely correlated with the causative Theileria spp. Thus, accurate detection and discrimination of Theileria spp. are essential for epidemiological studies and for provision of ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Small Ruminant ResearchAuthor(s): Alison M. Lee, Alan Wolfe, Joseph P. Cassidy, John Moriarty, Ronan O’Neill, Claire Fahy, Emily Connaghan, Chris Cousens, Mark P. Dagleish, Maire C. McElroy, Locksley L. McV. MessamAbstractJaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA). JSRV infection is usually detected post-mortem by macroscopic and histological examination of lungs for lesions of OPA. Subsequently, the presence of JSRV may be confirmed using polymerase chain reaction (PCR) on tumour tissue. Our goal was to d...
Source: Small Ruminant Research - Category: Zoology Source Type: research
This study not only represents the most systematic analysis of codon usage patterns in PEDV orf3 genes, but also provides a basic shaping mechanism of the codon usage bias.
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): Baolin Song, Qianmei Zeng, Yuan Liu, Bangyuan WuAbstractAbundant evidence now supports the idea that methionine deficiency has negative effects on chicken healthy especially in the aspect of cell cycle regulation and apoptosis. But, lacking of knowledge is the evaluation on metabolic organs. To test the effect of methionine deficiency on the kidney, we assessed the apoptosis and the cell cycle of kidney induced by methionine deficiency by the methods of TdT-mediated dUTP Nick-End Labeling (TUNEL), flow cytometry (FCM), quanti...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): Pascal Rainard
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): L.C. Rhades, M. Larzábal, A. Bentancor, J. Sabio y García, F.J. Babinec, A. Cataldi, N. Amigo, V.N. Baldone, L. Urquiza, P.J. Delicia, M.C. FortAbstractBovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July ...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
PMID: 31617895 [PubMed - in process]
Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research
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