Human exhaled air diagnostic markers for respiratory tract infections in subjects receiving mechanical ventilation

In this study we developed a noninvasive diagnosis method based on collecting non-volatile compounds in human exhaled air. We hypothesized that non-volatile compound profiles could be effectively used for bacterial RTI diagnosis. Exhaled air samples were collected from subjects receiving mechanical ventilation diagnosed with or without bacterial RTI in intensive care units at the Johns Hopkins Hospital. Truncated proteoforms, a class of non-volatile compounds, were characterized by top-down proteomics, and significant features associated with RTI were identified using feature selection algorithms. The results showed that three truncated proteoforms, collagen type VI alpha three chain protein, matrix metalloproteinase-9, and putative homeodomain transcription factor II were independently associated with RTI with the p-values of 2.0 ×

Source: Journal of Breath Research - January 19, 2023 Category: Respiratory Medicine Authors: Dapeng Chen, Marek A Mirski, Shuo Chen, Alese P Devin, Caroline R Haddaway, Emily R Caton, Wayne A Bryden and Michael McLoughlin Source Type: research