109. Use of Bionano Optical Genome Mapping in a multi-platform structural variation analysis of a cancer reference cell line

Genomic structural variation (SV) analysis is fundamental to understanding cancer genetics, but its detection remains elusive. Standard-of-care tests such as karyotyping are low-throughput and labor-intensive, while chromosomal microarrays (CMA) cannot find copy-neutral events. Advances in sequencing and Bionano optical genome mapping (OGM) can address these shortcomings. The OGM workflow includes extraction of megabases-long DNA, labeling at specific motifs, and linearization in nanochannels for imaging.
Source: Cancer Genetics and Cytogenetics - Category: Genetics & Stem Cells Authors: Source Type: research