Molecules, Vol. 27, Pages 7468: Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans

Molecules, Vol. 27, Pages 7468: Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans Molecules doi: 10.3390/molecules27217468 Authors: Carmen Tesoro Rosanna Ciriello Filomena Lelario Angela Di Capua Raffaella Pascale Giuliana Bianco Mario Dell’Agli Stefano Piazza Antonio Guerrieri Laura Scrano Sabino A. Bufo Maria Assunta Acquavia L-Dopa (LD), a substance used medically in the treatment of Parkinson’s disease, is found in several natural products, such as Vicia faba L., also known as broad beans. Due to its low chemical stability, LD analysis in plant matrices requires an appropriate optimization of the chosen analytical method to obtain reliable results. This work proposes an HPLC-UV method, validated according to EURACHEM guidelines as regards linearity, limits of detection and quantification, precision, accuracy, and matrix effect. The LD extraction was studied by evaluating its aqueous stability over 3 months. The best chromatographic conditions were found by systematically testing several C18 stationary phases and acidic mobile phases. In addition, the assessment of the best storage treatment of Vicia faba L. broad beans able to preserve a high LD content was performed. The best LD determination conditions include sun-drying storage, extraction in HCl 0.1 M, chromatographic separation with a Discovery C18 column, 250 × 4.6 mm, 5...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research