Analysis of isoalleles and flanking SNPs of STR markers by NGS to distinguish monozygotic twins

DNA analysis for forensic investigations is based on the concept that everyone is genetically unique, except in cases of monozygotic (MZ) twins. The most used markers are STRs, analyzed by CE, but they don ’t allow the differentiation of MZ twins. Human identification using NGS is already part of some forensic laboratories and it enables reading the STRs’ sequence and its isoalleles, but there is no reference to this application for differentiation of MZ twins. Herein, we evaluated the possibility to distinguish MZ using the Precision ID GlobalFilerTM NGS STR Panel v2.
Source: Forensic Science International: Genetics Supplement Series - Category: Forensic Medicine Authors: Source Type: research