An NGS-based microhaplotype system with high polymorphism for forensic DNA mixtures analysis

Microhaplotype is a novel genetic marker characterized by high polymorphism and short amplicon, which have a powerful potential in mixture deconvolution. The previous microhaplotype only contained single nucleotide polymorphisms (SNPs), we suggested two or more closely linked SNPs/insertion and deletion polymorphisms (Indels) associated in multiple allelic combinations within 300bp can be defined as generalized microhaplotypes to increase the number of candidate loci and polymorphism. We developed a system consisting of two groups of 62 and 71 microhaplotypes with high polymorphism based on the Next Generation Sequencing (NGS) platform for the mixture analysis.
Source: Forensic Science International: Genetics Supplement Series - Category: Forensic Medicine Authors: Source Type: research