An NGS-based microhaplotype system with high polymorphism for forensic DNA mixtures analysis
Microhaplotype is a novel genetic marker characterized by high polymorphism and short amplicon, which have a powerful potential in mixture deconvolution. The previous microhaplotype only contained single nucleotide polymorphisms (SNPs), we suggested two or more closely linked SNPs/insertion and deletion polymorphisms (Indels) associated in multiple allelic combinations within 300bp can be defined as generalized microhaplotypes to increase the number of candidate loci and polymorphism. We developed a system consisting of two groups of 62 and 71 microhaplotypes with high polymorphism based on the Next Generation Sequencing (NGS) platform for the mixture analysis.
Source: Forensic Science International: Genetics Supplement Series - Category: Forensic Medicine Authors: Mengyu Tan, Jiaming Xue, Ranran Zhrang, Hui Jian, Yuanyuan Xiao, Guihong Liu, Yazi Zheng, Qiushuo Wu, Shengqiu Qu, Weibo Liang Source Type: research